Background:  T lymphocytes express several low molecular mass transmembrane adaptor proteins that recruit SH2 domain-containing intracellular molecules to the cell membrane via tyrosine-based signaling pathways. One such protein, SIT (SHP2 interacting transmembrane adaptor protein) is a disulfide-linked homodimeric glycoprotein that is expressed in lymphocytes. SIT is reduced to half its molecular mass via endoglycosidase treatment. It contains five potential tyrosine phosphorylation sites, suggesting a role in TCR-mediated recruitment of SH2 domain-containing intracellular signaling molecules to the plasma membrane. SIT interacts with SHP2 and also with the adaptor protein Grb2. In addition, it is a substrate for the Src protein kinases Fyn, Lck and ZAP-70.

Description: Rabbit polyclonal to SIT

Immunogen: KLH conjugated synthetic peptide derived from SIT

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 21 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.