Background:  A variety of morphological and molecular changes are required for mature spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPANX (sperm protein associated with the nucleus, X-linked) family members are sperm- and testis-specific proteins containing between 97-103 amino acids, whose genes form a cluster on chromosome X. Sharing a high level of sequence similarity, SPANX-A, -B, -C, -D and -E localize to both cytoplasm and nucleus where they are associated with nuclear craters. SPANX-B (Sperm protein associated with the nucleus on the X chromosome B/F) is a 103 amino acid protein that is detected in round and elongating spermatids.

Description: Rabbit polyclonal to SPANXB1

Immunogen: KLH conjugated synthetic peptide derived from SPANXB1

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 12 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.