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Alpha crystallin antibody, α晶體蛋白抗體

 
編 號 PR-1643
產(chǎn)品名稱 Alpha crystallin antibody, α晶體蛋白抗體
規(guī) 格 0.2ml
價 格 1980元
應(yīng) 用 IHC,WB,ELISA,ICC,IP,IF
品 牌 Hopebiot

詳細(xì)信息: 說明書下載

Background:  Alpha crystallin is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of alpha crystallin is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, alpha crystallin is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Alpha crystallin does not increase after heat shock. The alpha crystallin was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. alpha crystallin may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of alpha crystallin is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions alpha crystallin is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Description: Rabbit polyclonal to Alpha crystallin

Immunogen: KLH conjugated synthetic peptide derived from Alpha crystallin

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 19 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.

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